Plant-pathogen interactions are strongly influenced by host developmental stage, yet many Arabidopsis thaliana-Botrytis cinerea assays remain centred on mature rosette leaves and rely on inoculum loads that may exceed those involved in a primary infection. We established a miniaturized in vitro A.…
Transposable elements (TEs) are powerful endogenous mutagens, but our understanding of the 'mobilome' - the set of mobile TEs at the species level - has largely been shaped by the study of a single reference genome. To overcome this reference-centric view, we produced long-read genome assemblies…
Phosphate starvation requires extensive metabolic and developmental reprogramming, yet how this response is coordinated with cellular energy status remains poorly understood. The energy sensor SnRK1 regulates plant growth and metabolism in response to energy availability, but its contribution to…
The cell wall and plasma membrane (PM) form the interface between a plant cell and its environment yet how their mechanical properties change in response to stress is poorly understood. Here we investigate how the mechanical properties of the cell wall, plasma membrane, and vacuole change in vivo…
Hemicellulosic xyloglucan is abundant in the primary cell walls of eudicots and non-gramineous monocots and contributes to their mechanical integrity. Its deficiency results in morphologically abnormal root hairs in Arabidopsis mutants lacking either two XYLOGLUCAN XYLOSYLTRANSFERASE (xxt1 xxt2) or…
Chloroplasts of land plants are dynamic organelles essential for photosynthesis, metabolism, and plant adaptation to changing environmental conditions. Within chloroplasts, the inner envelope and thylakoid membranes are spatially separated but functionally interconnected. The inner envelope harbors…
Reversible protein phosphorylation is a key regulator of chloroplast function. Although chloroplast kinases STN7, STN8, and plastid casein kinase 2 (pCK2) regulate photosynthetic acclimation and other chloroplast processes, their functional interplay remains poorly understood. We generated and…
Transient K+ efflux occurs rapidly following pathogen-associated molecular pattern (PAMP) perception by pattern recognition receptors (PRRs) as part of pattern-triggered immunity (PTI). However, the molecular identities of the K+ channels responsible for PTI-associated K+ efflux and the mechanisms…
DNA double-strand breaks (DSB) are highly toxic lesions that must be repaired to avoid major losses of genetic materials or genome rearrangements. A number of highly conserved molecular pathways are involved in DSB repair, each resulting in different repair outcomes for a given DSB. Over the past…